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Molecular cloning refers to the process of making multiple molecules.

Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA.

However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single stranded RNA or DNA production and a host of other molecular biology tools.

Nevertheless, these selection steps do not absolutely guarantee that the DNA insert is present in the cells obtained.

The reprogrammed cell begins to develop into an embryo because the egg reacts with the transferred nucleus.

The embryo will become genetically identical to the patient.

Further investigation of the resulting colonies must be required to confirm that cloning was successful.

This may be accomplished by means of PCR, restriction fragment analysis and/or DNA sequencing.

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